MBP antibody

Catalog No. ABIN6658054

Product Details anti-MBP Antibody

Target: MBP (Myelin Basic Protein (MBP))

Reactivity: Rat

Host: Chicken

Clonality: Polyclonal

Conjugate: This MBP antibody is un-conjugated

Application: Western Blotting (WB), Fluorescence Microscopy (FM)

Cross-Reactivity: Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): Cross reactivity with MBP from other mammalian tissues is expected.

Purification: Anti-MBP antibody is directed against Myelin basic protein.The antibody is a total IgY fraction. The antibody has been directly tested for reactivity in bovine, human, mouse and rat tissues.

Immunogen:

Immunogen: Anti-Myelin basic protein Antibody was produced in chicken by repeated immunizations with three peptide sequences conserved in higher vertebrate MBP proteins.

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Myelin Basic Protein antibody is suitable for use in Western Blotting and IF. Expect a bands of approximately 18 kDa and 22 kDa in size corresponding to MBP proteins in Western blots of rat brain homogenate. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:5000

IF Microscopy Dilution: 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.20.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Target Details for MBP

Target: MBP (Myelin Basic Protein (MBP))

Abstract: MBP Products

Synonyms: C76307 antibody, Hmbpr antibody, R75289 antibody, golli-mbp antibody, mld antibody, shi antibody, Mbps antibody, cb274 antibody, fj33b11 antibody, mbp antibody, wu:fj33b11 antibody, wu:fq15b02 antibody, zgc:136630 antibody, MGC115135 antibody, myelin basic protein antibody, myelin basic protein a antibody, myelin basic protein L homeolog antibody, MBP antibody, Mbp antibody, mbpa antibody, mbp antibody, mbp.L antibody

Background:

Synonyms: Myelin A1 protein, Myelin membrane encephalitogenic protein, Myelin basic protein, MBP, Myelin basic protein S, MBP S antibody

Background: Myelin Basic Protein antibody detects MBP. MBP is one of the major proteins of the myelin sheath surrounding axons in the nervous system. Since it is of relatively low molecular weight and high abundance the protein sequence was determined from purified protein over 30 years ago. The protein is made by oligodendrocytes in the central nervous system, so antibodies to MBP are good markers of this cell type. However, transcripts from the same gene are also expressed in certain hematopoetic lineage cells. In the central nervous system there are there different forms of the protein made by alternate transcription from a single gene, which have molecular weights of 21.5, 18.5, and 17.2 kDa. Since the two lower molecular weight forms are very close in molecular size, MBP antibodies typically show two bands on Western blots, one at about 22 kDa and another at about 18 kDa. Anti-MBP antibody is ideal for investigators involved in Cell Signaling, Neuroscience, and Signal Transduction research.

Gene Name: MDV1

Gene ID: 24547

NCBI Accession: NP_001020465

UniProt: P02688