SOD2 antibody

Catalog No. ABIN6658057

Supplier Product No.: 600-401-g13

The Rabbit Polyclonal anti-SOD2 antibody has been validated for WB, IHC, ELISA, IP and IF. It is suitable to detect SOD2 in samples from Rat.

Quick Overview for SOD2 antibody (ABIN6658057)

Target: SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SOD2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF)

Product Details anti-SOD2 Antibody

Supplier Product No.: 600-401-g13

Supplier: Rockland

Purpose: SOD2 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with SOD2 from Human, Rat, Mouse, Bovine, Canine, Chicken, Drosophila, Guinea pig, Pig, Hamster, Monkey, Rabbit, Sheep, and Xenopus based on 100 % homology with the immunizing sequence.

Purification: Anti-SOD2 Antibody was purified by affinity chromatography.

Sterility: Sterile filtered

Immunogen: SOD2 Antibody was produced from whole rabbit serum prepared by repeated immunizations raised against rat SOD2.

Isotype: IgG

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

ELISA_Dilution: 1:200

Immunohistochemistry_Dilution: User Optimized

Western_Blot_Dilution: 0.5 μg/mL

Comment:

Anti-SOD2 Antibody is tested for use in WB, IP, IF, and IHC. Expect a band approximately ~25kDa protein corresponding to the molecular mass of SOD2 in WB. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.1 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for SOD2

Target: SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))

Alternative Name: Mn SOD

Background:

Synonyms: Manganese SOD, IPO B, Mn SOD, SOD2, Superoxide dismutase [Mn], mitochondrial

Background: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body. It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase. In general, SODs play a major role in antioxidant defense mechanisms. There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space. SOD3 can also be distinguished by its heparin-binding capacity.

Gene Name: Sod2

Gene ID: 24787

NCBI Accession: NP_058747

UniProt: P07895

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis, Negative Regulation of intrinsic apoptotic Signaling