NMDAR2A antibody (C-Term)

Catalog No. ABIN6658061

Supplier Product No.: 600-401-c86

This anti-NMDAR2A antibody is a Rabbit Polyclonal antibody detecting NMDAR2A in WB, IHC and IP. Suitable for Rat.

Quick Overview for NMDAR2A antibody (C-Term) (ABIN6658061)

Target: NMDAR2A (GRIN2A) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NMDAR2A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-NMDAR2A Antibody

Binding Specificity: C-Term

Supplier Product No.: 600-401-c86

Supplier: Rockland

Purpose: NMDA NR2A Subunit Antibody

Cross-Reactivity (Details): Anti-NMDA NR2A Subunit antibody is directed against NMDA NR2A protein.

Purification: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: Anti-NMDA NR2A subunit Antibody was produced by repeated immunizations with a fusion protein from the C-terminal region of the NR2A subunit.

Isotype: IgG

Application Details

Application Notes:

Immunoprecipitation_Dilution: 3 μL per 200 μg lysate

Immunohistochemistry_Dilution: 1:1000-1:2000

Western_Blot_Dilution: 1:1000

Other: User Optimized

Comment:

Anti-NMDA NR2A Subunit (Rabbit) Antibody is tested for use in Western Blotting, Immunoprecipitation and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 180 kDa in size corresponding to the NR2A subunit of the NMDA receptor. This antibody is lyophilized from 5 mM ammonium bicarbonate.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Neutral PBS

Reconstitution_Volume: 50μL

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for NMDAR2A

Target: NMDAR2A (GRIN2A) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A))

Alternative Name: NMDA NR2A Subunit

Background:

Synonyms: Glutamate [NMDA] receptor subunit epsilon-1, N-methyl D-aspartate receptor subtype 2A, NMDAR2A, NR2A, Grin2a

Background: NMDA NR2A Subunit antibody detects NMDA NR2A protein. The ion channels activated by glutamate are typically divided into two classes. Glutamate receptors that are activated by kainate and α-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death. The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS. The NMDAR is also potentiated by protein phosphorylation. The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits. Anti-NMDA NR2A Subunit Antibody is ideal for investigators involved in Neuroscience and Signal Transduction research.

Gene Name: GRIN2A

Gene ID: 24409

NCBI Accession: NP_036705

UniProt: Q00959

Pathways: Synaptic Membrane, Regulation of long-term Neuronal Synaptic Plasticity