GRIA2 antibody (C-Term)

Catalog No. ABIN6658073

Supplier Product No.: 200-301-f34

This anti-GRIA2 antibody is a Mouse Monoclonal antibody detecting GRIA2 in WB, IHC, IP and FM. Suitable for Rat.

Quick Overview for GRIA2 antibody (C-Term) (ABIN6658073)

Target: GRIA2 (Glutamate Receptor, Ionotropic, AMPA 2 (GRIA2))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This GRIA2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Clone: S21-32

Product Details anti-GRIA2 Antibody

Binding Specificity: C-Term

Supplier Product No.: 200-301-f34

Supplier: Rockland

Purpose: GluR2 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with GluR2 from Human, Mouse, and Rat based on 100 % homology with the immunizing sequence.

Purification: Anti-GluR2 Antibody was purified by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: GluR2 Antibody was produced in mice by repeated immunizations with a fusion protein corresponding to a cytoplasmic C-terminus region of rat GluR2.

Isotype: IgG1

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: 0.1-1.0 μg/mL

IF_Microscopy_Dilution: 1.0-10 μg/mL

Western_Blot_Dilution: 1 μg/mL

Comment:

Anti-GluR2 Antibody is tested for use in WB and IHC, and is suitable in IP and Immuno-gold EM. Expect a band approximately ~90kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for GRIA2

Target: GRIA2 (Glutamate Receptor, Ionotropic, AMPA 2 (GRIA2))

Alternative Name: GluR2

Background:

Synonyms: Glutamate receptor 2, GluR-2, GLUR2, AMPA-selective glutamate receptor 2, GluR-B, GluR-K2, Glutamate receptor ionotropic, AMPA 2, GluA2

Background: The AMPA receptor is a non-NMDA-type ionotropic transmembrane receptor for glutamate that mediates fast synaptic transmission in the CNS. AMPARs are composed of four types of subunits, designated as GluR1, GluR2, GluR3 and GluR4, which combine to form tetramers. GluR2 is possibly the most important AMPA receptor subunit, responsible for AMPA receptor rectifying properties, control of ion flow and in particular, the influx of calcium. The majority of GluR2 in the CNS is expressed in the GluR2 form, containing a critical arginine residue in the transmembrane region 2 domain, thereby rendering native AMPA receptors impermeable to calcium.

Gene Name: GRIA2

Gene ID: 29627

UniProt: P19491

Pathways: PI3K-Akt Signaling