Tyrosine Hydroxylase antibody

Catalog No. ABIN6658149

Supplier Product No.: 600-601-c98

Product Details anti-Tyrosine Hydroxylase Antibody

Target: Tyrosine Hydroxylase (TH)

Reactivity: Rat

Host: Sheep

Clonality: Polyclonal

Conjugate: This Tyrosine Hydroxylase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Supplier Product No.: 600-601-c98

Supplier: Rockland

Purpose: Tyrosine Hydroxylase Antibody

Cross-Reactivity (Details): Anti-Tyrosine Hydroxylase Antibody is directed against rat Tyrosine Hydroxylase protein.

Purification: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: Anti-Tyrosine Hydroxylase Antibody was produced by repeated immunizations with SDS-denatured, native rat tyrosine hydroxylase purified from pheochromocytoma.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: 1:1000

IF_Microscopy_Dilution: 1:1000

Western_Blot_Dilution: 1:1000

Other: User Optimized

Comment:

Anti-Tyrosine Hydroxylase (Sheep) antibody is tested for use in Western Blotting, IHC and ICC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 60 kDa in size corresponding to tyrosine hydroxylase protein in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Tyrosine Hydroxylase

Target: Tyrosine Hydroxylase (TH)

Alternative Name: Tyrosine Hydroxylase (TH Products)

Background:

Synonyms: Tyrosine 3-monooxygenase, Tyrosine 3-hydroxylase, TH

Background: Tyrosine Hydroxylase Antibody detects Tyrosine Hydroxylase protein. Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse. TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling.

Gene Name: TH

Gene ID: 25085

NCBI Accession: NP_036872

UniProt: P04177

Pathways: Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour