GRM7 antibody (pSer862)

Catalog No. ABIN6658162

Product Details anti-GRM7 Antibody

Target: GRM7 (Glutamate Receptor, Metabotropic 7 (GRM7))

Binding Specificity: pSer862

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRM7 antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): Cross reactivity with mGluR7 from other species has not been determined.

Purification: Anti mGluR7 pS862 antibodiy is directed against mGluR7 phosphorylated at Ser862. GluR7 antibdody is affinity purified from monospecific antiserum by immunoaffinity purification. Immunolabeling is blocked by preadsorption of antibody with the phospho-peptide used as antigen but not by the corresponding dephosphopeptide. The antibody is a total IgG fraction. Expect reactivity with the following species based on sequence homology: bovine, canine, human, non-human primate and zebrafish.

Immunogen:

Immunogen: Anti-mGluR7 pS862 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser 862 conjugated to KLH.

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-mGluR7 pS862 (Rabbit) antibody is suitable for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 102 kDa in size corresponding to the mGluR7 protein phosphorylated at Ser862 in the appropriate cell lysate or extract.

Western Blot Dilution: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Target Details for GRM7

Target: GRM7 (Glutamate Receptor, Metabotropic 7 (GRM7))

Alternative Name: mGluR7 (GRM7 Products)

Synonyms: MGLUR7 antibody, GLUR7 antibody, GPRC1G antibody, MGLU7 antibody, 6330570A01Rik antibody, BB176677 antibody, C030018L03 antibody, E130018M02Rik antibody, Gpr1g antibody, Gprc1g antibody, mGluR7 antibody, glutamate metabotropic receptor 7 antibody, glutamate receptor, metabotropic 7 antibody, GRM7 antibody, Grm7 antibody

Background:

Synonyms: mGluR7, Metabotropic Glutamate Receptor 7

Background: mGluR7 pS862 antibodiy detects mGluR7 when phosphorylated at Ser862. Metabotropic glutamate receptors (mGluRs) are key receptors in the modulation of excitatory synaptic transmission in the central nervous system. They are implicated in many forms of neural plasticity as well as learning and memory and drug abuse. The mGluRs are divided into three groups based on sequence identity and pharmacological properties: group I (mGluR1 and mGluR5) are localized in the perisynaptic region of the postsynaptic membrane, whereas group II (mGlur2 and mGluR3) and group III (mGluR4,6,7 and 8) are localized predominantly at presynaptic terminals. PKC phosphorylation of serine 862 on mGluR7 has been shown to be critical for stabilizing receptor surface expression and promoting binding to the synaptic PDZ-domain-containing protein PICK1. Therefore, mGluR7 pS862 antibody is ideal for investigators involved in neuronal circuitry and more generally in Neuroscience.

Gene Name: GRM7

Gene ID: 81672

UniProt: P35400

Pathways: Sensory Perception of Sound, cAMP Metabolic Process, Feeding Behaviour