SYN1 antibody (pSer9)

Catalog No. ABIN6658201

Supplier Product No.: 612-401-c93

The Rabbit Polyclonal anti-SYN1 antibody has been validated for WB and IHC. It is suitable to detect SYN1 in samples from Rat.

Quick Overview for SYN1 antibody (pSer9) (ABIN6658201)

Target: SYN1 (Synapsin I (SYN1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SYN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SYN1 Antibody

Binding Specificity: pSer9

Supplier Product No.: 612-401-c93

Supplier: Rockland

Purpose: Synapsin I phospho S9 Antibody

Cross-Reactivity (Details): The antibody detects the phosphorylated S9 amino acid.

Purification: Anti-Synapsin I pS9 antibody is directed against rat Synapsin I.

Immunogen: Anti-Synapsin I pS9 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser9 of Synapsin I.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: 1:500

Western_Blot_Dilution: 1:1000

Comment:

Anti-Synapsin I pS9 (Rabbit) antibody is tested for use in Western Blotting and ICC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 78 kDa in size corresponding to synapsin I doublet protein phosphorylated at Ser9 in the appropriate cell lysate or extract. This antibody also weakly labels the approximately 55 kDa synapsin II protein which has a similar phosphorylation site to that of Ser9 on synapsin I. Researchers should determine optimal titers for applications that are not stated below.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for SYN1

Target: SYN1 (Synapsin I (SYN1))

Alternative Name: Synapsin I

Background:

Synonyms: Synapsin I, SYN1

Background: Synapsin I pS9 antibody is directed against rat Synapsin I, which plays a key role in synaptic plasticity in brain. This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. In addition to its role in plasticity, the expression of synapsin I is a precise indicator of synapse formation. Thus, synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis. The role of synapsin in synaptic plasticity and in synaptogensis is regulated by phosphorylation. Serine 9 is the site on synapsin I that is phosphorylated by cAMP-dependent protein kinase and by calcium calmodulin kinase I. Phosphorylation of this site is thought to regulate synaptic vesicle function and neurite outgrowth. Anti-Synapsin I pS9 Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Gene Name: SYN1

Gene ID: 24949

NCBI Accession: NP_001104252

UniProt: P09951