GABRG2 antibody (pSer327)

Catalog No. ABIN6658228

Product Details anti-GABRG2 Antibody

Target: GABRG2 (gamma-aminobutyric Acid (GABA) A Receptor, gamma 2 (GABRG2))

Binding Specificity: pSer327

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABRG2 antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): Cross reactivity with GABA(A) Receptor gamma 2 pS327 from other sources has not been determined

Purification: Anti-GABA(A) Receptor gamma 2 pS327 Antibody is directed against rat phosphorylated GABA(A) Receptor gamma 2. The antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Immunolabeling of the GABAA band is completely blocked by lambda-phosphatase treatment. This antibody is directed against rat GABA(A) receptor gamma 2 proteins phosphorylated at Ser327. . However, reactivity is also expected against bovine, canine, chicken, human, and non-human primate.

Immunogen:

Immunogen: Anti-GABA(A) Receptor gamma 2 pS327 Antibody was produced by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser327.

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-GABA(A) Receptor gamma 2 pS327 (Rabbit) antibody is suitable for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 45 kDa in size corresponding to GABA(A) receptor gamma 2 subunit phosphorylated at Ser327 in the appropriate cell lysate or extract.

Western Blot Dilution: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Target Details for GABRG2

Target: GABRG2 (gamma-aminobutyric Acid (GABA) A Receptor, gamma 2 (GABRG2))

Alternative Name: GABA(A) Receptor gamma 2 (GABRG2 Products)

Synonyms: cae2 antibody, eca2 antibody, gabrg1 antibody, gefsp3 antibody, si:ch211-145n14.1 antibody, CAE2 antibody, ECA2 antibody, GEFSP3 antibody, GABAA-R antibody, Gabrg-2 antibody, gamma2 antibody, gamma-aminobutyric acid type A receptor gamma2 subunit antibody, gamma-aminobutyric acid (GABA) A receptor, gamma 2 antibody, gamma-aminobutyric acid type A receptor gamma 2 subunit antibody, gamma-aminobutyric acid (GABA) A receptor, subunit gamma 2 antibody, GABRG2 antibody, gabrg2 antibody, Gabrg2 antibody

Background:

Synonyms: Gamma-aminobutyric acid receptor subunit gamma-2, GABA(A) receptor subunit gamma-2, Gabrg2

Background: Anti-GABA(A) Receptor gamma 2 pS327 Antibody detects phosphorylated GABA(A) Receptor gamma 2. Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABAA and the GABAB subtype of receptors. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and sub-stance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified. Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma- subunit is required for benzodiazepine modulation. It has recently been suggested that PKC? regulates the sensitivity of GABAA alpha1-beta2-gamma2 receptors to ethanol and benzodiazepines through phosphorylation of serine 327 in the large intracellular loop of gamma2. GABA(A) Receptor gamma2 pS327 antibody is ideal for investigators involved in Neuroscience.

Gene Name: GABRG2

Gene ID: 29709, 8393403

UniProt: P18508