Connexin 43/GJA1 antibody (pSer368)

Catalog No. ABIN6658233

Supplier Product No.: 612-401-d23

This Rabbit Polyclonal antibody specifically detects Connexin 43/GJA1 in WB and ELISA. It exhibits reactivity toward Rat.

Quick Overview for Connexin 43/GJA1 antibody (pSer368) (ABIN6658233)

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Connexin 43/GJA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Connexin 43/GJA1 Antibody

Binding Specificity: pSer368

Supplier Product No.: 612-401-d23

Supplier: Rockland

Purpose: Connexin 43 phospho S368 Antibody

Cross-Reactivity (Details): Anti-Connexin 43 pS368 antibody is directed against rat pS368 Connexin 43

Purification: .Anti-Connexin 43 pS368 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Immunogen: Anti-Connexin 43 pS368 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser368 conjugated to KLH.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000

Western_Blot_Dilution: 1:1000

Comment:

Anti-Connexin 43 pS368 Antibody has been tested in western blot and is suitable for use in ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 43 kDa in size corresponding to connexin 43 protein phosphorylated at Ser368 in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Connexin 43/GJA1

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Alternative Name: Connexin 43

Background:

Synonyms: Gap junction alpha-1 protein, Gap junction 43 kDa heart protein, CX43

Background: Anti-Connexin 43 pS368 Antibody detects pS368 Connexin which is thought to play a key role in development and may also be involved in epilepsy. Connexin 43 antibody recognizes Connexin43 which forms gap-junctional channels and regulates the permeability of these gap junctions to small organic molecules. Permeability of connexin43 is known to be regulated by phosphorylation at Ser368 by protein kinase C. Phosphorylation of Ser368 by PKC induces a conformational change of connexin 43 that results in a decrease in gap junction permeability. Anti-Connexin 43 pS368 Antibody is ideal for investigators involved in Neuroscience and Cell Signaling.

Gene Name: GJA1

Gene ID: 24392

UniProt: P08050

Pathways: MAPK Signaling, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization