Tyrosine Hydroxylase antibody (pSer31)

Catalog No. ABIN6658241

Supplier Product No.: 600-401-e51

This anti-Tyrosine Hydroxylase antibody is a Rabbit Polyclonal antibody detecting Tyrosine Hydroxylase in WB, IHC and FM. Suitable for Rat.

Quick Overview for Tyrosine Hydroxylase antibody (pSer31) (ABIN6658241)

Target: Tyrosine Hydroxylase (TH)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tyrosine Hydroxylase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Product Details anti-Tyrosine Hydroxylase Antibody

Binding Specificity: pSer31

Supplier Product No.: 600-401-e51

Supplier: Rockland

Purpose: Tyrosine Hydroxylase phospho S31 Antibody

Cross-Reactivity (Details): Anti-Tyrosine Hydroxylase pS31 Antibody is directed against phosphorylated rat Tyrosine Hydroxylase protein.

Purification: Tyrosine Hydroxylase pS31 antibodies are affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: Anti-Tyrosine Hydroxylase pS31 Antibody was produced repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser31.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: 1:1000

IF_Microscopy_Dilution: 1:1000

Western_Blot_Dilution: 1:1000

Other: User Optimized

Comment:

Anti-Tyrosine Hydroxylase pS31 (Rabbit) antibody is tested for use in Western Blotting, IHC and ICC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 60 kDa in size corresponding to tyrosine hydroxylase protein phosphorylated at Ser31 in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Tyrosine Hydroxylase

Target: Tyrosine Hydroxylase (TH)

Alternative Name: Tyrosine Hydroxylase

Background:

Synonyms: Tyrosine 3-monooxygenase, Tyrosine 3-hydroxylase, TH

Background: Tyrosine Hydroxylase pS31 Antibody detects phosphorylated Tyrosine Hydroxylase protein. Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse. TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling. The activity of TH is also regulated by phosphorylation. Phospho-specific antibodies for the phosphorylation sites on TH can be used to great effect in studying this regulation and in identifying the cells in which TH phosphorylation occurs.

Gene Name: TH

Gene ID: 25085

UniProt: P04177

Pathways: Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour