SYN1 antibody (pSer549)

Catalog No. ABIN6658253

Supplier Product No.: 612-401-c94

This anti-SYN1 antibody is a Rabbit Polyclonal antibody detecting SYN1 in WB and IHC. Suitable for Rat.

Quick Overview for SYN1 antibody (pSer549) (ABIN6658253)

Target: SYN1 (Synapsin I (SYN1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SYN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SYN1 Antibody

Binding Specificity: pSer549

Supplier Product No.: 612-401-c94

Supplier: Rockland

Purpose: Synapsin I phospho S549 Antibody

Cross-Reactivity (Details): Anti-Synapsin I pS549 antibody is directed against rat Synapsin I.

Purification: Synapsin I pS549 was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: Anti-Synapsin I pS549 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser549 of Synapsin I.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: 1:500

Western_Blot_Dilution: 1:1000

Comment:

Suggested Applications: Titrated value sufficient to run approximately 10 mini blots.
Anti-Synapsin pS549 (Rabbit) antibody is tested for use in Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 78 kDa in size corresponding to synapsin I doublet phosphorylated at Ser549 in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for SYN1

Target: SYN1 (Synapsin I (SYN1))

Alternative Name: Synapsin I

Background:

Synonyms: Synapsin I, SYN1

Background: Synapsin I pS549 antibody is directed against rat Synapsin I, which plays a key role in synaptic plasticity in brain. This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. The role of synapsin in synaptic plasticity and in synaptogensis is regulated by phosphorylation. Ser 549 along with Ser 62 and Ser 67 are the sites of synapsin I that are phosphorylated by MAP kinase. Phosphorylation and subsequent dephosphorylation of this site is thought to play a key role in synaptic vesicle trafficking. Anti-Synapsin I pS549 Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Gene Name: SYN1

Gene ID: 24949

NCBI Accession: NP_001104252

UniProt: P09951