Tryptophan Hydroxylase 2 antibody (pSer19)

Catalog No. ABIN6658283

Supplier Product No.: 612-401-e48

This anti-Tryptophan Hydroxylase 2 antibody is a Rabbit Polyclonal antibody detecting Tryptophan Hydroxylase 2 in WB. Suitable for Rat.

Quick Overview for Tryptophan Hydroxylase 2 antibody (pSer19) (ABIN6658283)

Target: Tryptophan Hydroxylase 2 (TPH2)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tryptophan Hydroxylase 2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Tryptophan Hydroxylase 2 Antibody

Binding Specificity: pSer19

Supplier Product No.: 612-401-e48

Supplier: Rockland

Purpose: Tryptophan Hydroxylase 2 phospho S19 Antibody

Cross-Reactivity (Details): Anti-Tryptophan Hydroxylase 2 is directed against rat Tryptophan Hydroxylase phosphorylated at Ser19.

Purification: Tryptophan Hydroxylase 2 pS19 antibodies are prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Immunogen: Anti-Tryptophan Hydroxylase 2 pS19 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser19 of Tryptophan Hydroxylase.

Isotype: IgG

Application Details

Application Notes: Optional[Neutralization_Dilution]: 1:1000

Comment:

Anti-Trytophan Hydroxylase2 pS19 (Rabbit) antibody is tested for use in Western Blotting. Expect a band of approximately 55 kDa in size corresponding to tryptophan hydroxylase protein phosphorylated at Ser 19 in the appropriate cell lysate or extract. Researchers should determine optimal titers for applications that are not stated below.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Tryptophan Hydroxylase 2

Target: Tryptophan Hydroxylase 2 (TPH2)

Alternative Name: Tryptophan Hydroxylase 2

Background:

Synonyms: Tryptophan 5-hydroxylase 2, Neuronal tryptophan hydroxylase, Tryptophan 5-monooxygenase 2, TPH

Background: Tryptophan Hydroxylase 2 pS19 antibody detects TPH2 protein. Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II). CaM K II phosphorylates Ser19 which lies within the regulatory domain of TPH2. Anti-TPH2pS19 antibody is ideal for investigators involved in Cell Signaling and Neuroscience research.

Gene Name: TPH2

Gene ID: 317675

UniProt: Q8CGU9