Donkey anti-Goat IgG Antibody (DyLight 800)

Catalog No. ABIN6698878

Supplier Product No.: 605-745-002

Quick Overview for Donkey anti-Goat IgG Antibody (DyLight 800) (ABIN6698878)

Target: IgG

Clonality: Polyclonal

Conjugate: DyLight 800

Application: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)

Isotype: IgG

Product Details

Reactivity: Goat

Host: Donkey

Supplier Product No.: 605-745-002

Supplier: Rockland

Purpose: Goat IgG (H&L) Antibody DyLight™ 800 Conjugated

Characteristics: Donkey anti-Goat IgG Antibody DyLight™ 800 Conjugated Pre-Adsorbed, Donkey anti-Goat IgG DyLight™ 800 Conjugated Antibody,Anti-Goat IgG DyLight Antibody generated in donkey detects goat IgG.

Purification:

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Goat IgG and Goat Serum.  This antibody will react with heavy chains of Goat IgG and with light chains of most Goat immunoglobulins.

Immunogen: Goat IgG, whole molecule

Labeling Ratio: 2.02

Application Details

Application Notes:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000

Other: User Optimized

Comment:

Anti-Goat IgG DyLight 800 Antibody has been tested by dot blot. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Suggested Applications: WB

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References

Downey-Kopyscinski, Daily, Gautier, Bhatt, Florea, Mitsiades, Richardson, Driessen, Overkleeft, Kisselev: "An inhibitor of proteasome β2 sites sensitizes myeloma cells to immunoproteasome inhibitors." in: Blood advances, Vol. 2, Issue 19, pp. 2443-2451, (2018) (PubMed).

Target Details

Target: IgG

Target Type: Antibody

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.