Cyclin D3 antibody (AA 121-220) (Cy5)

Catalog No. ABIN671575

This Rabbit Polyclonal antibody specifically detects Cyclin D3 in WB, IF (cc), IF (p) and FACS. It exhibits reactivity toward Human and Rabbit.

Quick Overview for Cyclin D3 antibody (AA 121-220) (Cy5) (ABIN671575)

Target: Cyclin D3 (CCND3)

Reactivity: Human, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin D3 antibody is conjugated to Cy5

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS)

Product Details anti-Cyclin D3 Antibody (Cy5)

Binding Specificity: AA 121-220

Cross-Reactivity: Human, Rabbit

Predicted Reactivity: Mouse,Rat,Dog,Cow,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Cyclin D3

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Cyclin D3

Target: Cyclin D3 (CCND3)

Alternative Name: Cyclin D3

Background:

Synonyms: G1/S-specific cyclin-D3, CCND3

Background: Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.

Gene ID: 896

UniProt: P30281

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, Glycosaminoglycan Metabolic Process