Cortactin antibody (AA 1-105)

Catalog No. ABIN6719306

The Rabbit Polyclonal anti-Cortactin antibody has been validated for WB, IHC, ELISA, IF, ICC and FACS. It is suitable to detect Cortactin in samples from Human, Mouse and Rat.

Quick Overview for Cortactin antibody (AA 1-105) (ABIN6719306)

Target: Cortactin (CTTN)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cortactin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-Cortactin Antibody

Binding Specificity: AA 1-105

Purpose: Anti-Cortactin/CTTN Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Cortactin/CTTN Antibody Picoband® (ABIN6719306). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human Cortactin recombinant protein (Position: M1-Q105).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
Immunocytochemistry, 0.5-1 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
Immunofluorescence, 2 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Tegtmeyer, N., Wittelsberger, R., Hartig, R., Wessler, S., Martinez-Quiles, N., Backert, S. Serine phosphorylation of cortactin controls focal adhesion kinase activity and cell scattering induced by Helicobacter pylori. Cell Host Microbe 9: 520-531, 2011. 2. Williams, M. R., Markey, J. C., Doczi, M. A., Morielli, A. D. An essential role for cortactin in the modulation of the potassium channel Kv1.2. Proc. Nat. Acad. Sci. 104: 17412-17417, 2007.

Comment:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Cortactin

Target: Cortactin (CTTN)

Alternative Name: CTTN

Background:

Synonyms: Src substrate cortactin, Amplaxin, Oncogene EMS1, CTTN, EMS1

Background: Cortactin, mapped to 11q13.3, is a multidomain protein. This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for this gene.

Molecular Weight: 85 kDa

UniProt: Q14247

Pathways: MAPK Signaling