ERCC3 antibody (AA 665-715)

Catalog No. ABIN6719310

This anti-ERCC3 antibody is a Rabbit Polyclonal antibody detecting ERCC3 in WB and ELISA. Suitable for Human, Rat and Mouse.

Quick Overview for ERCC3 antibody (AA 665-715) (ABIN6719310)

Target: ERCC3 (DNA Repair Protein Complementing XP-B Cells (ERCC3))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERCC3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ERCC3 Antibody

Binding Specificity: AA 665-715

Purpose: Anti-XPB/ERCC3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-XPB/ERCC3 Antibody Picoband® (ABIN6719310). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human XPB recombinant protein (Position: Q665-K715).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Coin, F., Oksenych, V., Egly, J.-M. Distinct roles for the XPB/p52 and XPD/p44 subcomplexes of TFIIH in damaged DNA opening during nucleotide excision repair. Molec. Cell 26: 245-256, 2007. 2. Kim, T.-K., Ebright, R. H., Reinberg, D. Mechanism of ATP-dependent promoter melting by transcription factor IIH. Science 288: 1418-1421, 2000. 3. Le May, N., Dubaele, S., De Santis, L. P., Billecocq, A., Bouloy, M., Egly, J.-M.TFIIH transcription factor, a target for the Rift Valley hemorrhagic fever virus. Cell 116: 541-550, 2004.

Comment:

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ERCC3

Target: ERCC3 (DNA Repair Protein Complementing XP-B Cells (ERCC3))

Alternative Name: ERCC3

Background:

Synonyms: General transcription and DNA repair factor IIH helicase subunit XPB, TFIIH subunit XPB, Basic transcription factor 2 89 kDa subunit, BTF2 p89, DNA excision repair protein ERCC-3, DNA repair protein complementing XP-B cells, TFIIH basal transcription factor complex 89 kDa subunit, TFIIH 89 kDa subunit, TFIIH p89, Xeroderma pigmentosum group B-complementing protein, ERCC3, XPB, XPBC

Tissue Specificity: Mainly expressed in pachytene spermatocytes of testis and in lymphocyte-rich areas of spleen and lymph nodes. Isoform v1 is expressed in spleen. Isoform v2 is expressed in testis. Also detected in ovary, placenta, pancreas, cardiac fibroblasts. Expressed in B-cells and prostate malignant cells. Isoform v1 and isoform v3 are expressed in epithelial colorectal adenocarcinoma cells. Isoform v2 and isoform v4 are expressed in endothelial cells. Isoform v1, isoform v2, isoform v3 and isoform v4 are expressed in pulmonary artery smooth muscle cells. Isoform v2 and isoform v5 are expressed in microvascular endothelial cells (at protein level).

Background: XPB (xeroderma pigmentosum type B) is an ATP-dependent DNA helicase in humans that is a part of the TFIIH transcription factor complex. This gene encodes an ATP-dependent DNA helicase that functions in nucleotide excision repair. The encoded protein is a subunit of basal transcription factor 2 (TFIIH) and, therefore, also functions in class II transcription. Mutations in this gene are associated with Xeroderma pigmentosum B, Cockayne's syndrome, and trichothiodystrophy. Alternative splicing results in multiple transcript variants.

Molecular Weight: 89 kDa

Gene ID: 2071

UniProt: P19447

Pathways: DNA Damage Repair