NLRP2 antibody (AA 9-190)

Catalog No. ABIN6719345

The Rabbit Polyclonal anti-NLRP2 antibody has been validated for WB and ELISA. It is suitable to detect NLRP2 in samples from Human, Mouse and Rat.

Quick Overview for NLRP2 antibody (AA 9-190) (ABIN6719345)

Target: NLRP2 (NLR Family, Pyrin Domain Containing 2 (NLRP2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NLRP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-NLRP2 Antibody

Binding Specificity: AA 9-190

Purpose: Anti-NALP2/NLRP2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NALP2/NLRP2 Antibody Picoband® (ABIN6719345). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NALP2/NLRP2 recombinant protein (Position: F9-K190).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Kuchmiy, A. A., D'Hont, J., Hochepied, T., Lamkanfi, M. NLRP2 controls age-associated maternal fertility. J. Exp. Med. 213: 2851-2860, 2016. 2. Martinon, F., Hofmann, K., Tschopp, J. The pyrin domain: a possible member of the death domain-fold family implicated in apoptosis and inflammation. Curr. Biol. 10: R118-R120, 2001. 3. Peng, H., Chang, B., Lu, C., Su, J., Wu, Y., Lv, P., Wang, Y., Liu, J., Zhang, B., Quan, F., Guo, Z., Zhang, Y. Nlrp2, a maternal effect gene required for early embryonic development in the mouse. PLoS One 7: e30344, 2012.

Comment:

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for NLRP2

Target: NLRP2 (NLR Family, Pyrin Domain Containing 2 (NLRP2))

Alternative Name: NLRP2

Background:

Synonyms: NACHT, LRR and PYD domains-containing protein 2, Nucleotide-binding site protein 1, PYRIN domain and NACHT domain-containing protein 1, PYRIN-containing APAF1-like protein 2, NLRP2, NALP2, NBS1, PAN1, PYPAF2

Tissue Specificity: Expressed at high levels in lung, placenta and thymus and at lower levels in ovary, intestine and brain.

Background: NACHT, LRR and PYD domains-containing protein 2 is a protein that in humans is encoded by the NLRP2 gene. It is mapped to 19q13.42. This gene is a member of the nucleotide-binding and leucine-rich repeat receptor (NLR) family, and is predicted to contain an N-terminal pyrin effector domain (PYD), a centrally-located nucleotide-binding and oligomerization domain (NACHT) and C-terminal leucine-rich repeats (LRR). Members of this gene family are thought to be important regulators of immune responses. This gene product interacts with components of the IkB kinase (IKK) complex, and can regulate both caspase-1 and NF-kB (nuclear factor kappa-light-chain-enhancer of activated B cells) activity. The pyrin domain is necessary and sufficient for suppression of NF-kB activity. An allelic variant (rs147585490) has been found that is incapable of blocking the transcriptional activity of NF-kB. Alternative splicing results in multiple transcript variants encoding different isoforms.

Molecular Weight: 121 kDa

Gene ID: 55655

Pathways: Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Inflammasome