TXN antibody (AA 2-105)

Catalog No. ABIN6719381

The Rabbit Polyclonal anti-TXN antibody has been validated for WB, IHC, ELISA, ICC and IF. It is suitable to detect TXN in samples from Rat and Mouse.

Quick Overview for TXN antibody (AA 2-105) (ABIN6719381)

Target: TXN (Thioredoxin (TXN))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TXN antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-TXN Antibody

Binding Specificity: AA 2-105

Purpose: Anti-Thioredoxin/TRX/Txn Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Thioredoxin/TRX/Txn Antibody Picoband® (ABIN6719381). Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived mouse Thioredoxin TRX recombinant protein (Position: V2-A105).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Benhar, M., Forrester, M. T., Hess, D. T., Stamler, J. S. Regulated protein denitrosylation by cytosolic and mitochondrial thioredoxins. Science 320: 1050-1054, 2008. 2. Im, J.-Y., Lee, K.-W., Woo, J.-M., Junn, E., Mouradian, M. M. DJ-1 induces thioredoxin 1 expression through the Nrf2 pathway. Hum. Molec. Genet. 21: 3013-3024, 2012.

Comment:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for TXN

Target: TXN (Thioredoxin (TXN))

Alternative Name: Txn

Background:

Synonyms: Thioredoxin, Trx, ATL-derived factor, ADF, Txn, Txn1

Tissue Specificity: Colon, small intestine, colonic tumors, bronchus, cervix and gall bladder.

Background: Thioredoxin is a class of small redox proteins known to be present in all organisms. It is mapped to 9q31.3. The protein encoded by this gene acts as a homodimer and is involved in many redox reactions. The encoded protein is active in the reversible S-nitrosylation of cysteines in certain proteins, which is part of the response to intracellular nitric oxide. This protein is found in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 12 kDa

Gene ID: 22166

UniProt: P10639

Pathways: Carbohydrate Homeostasis, Cell RedoxHomeostasis