Integrin Alpha2b antibody (AA 32-263) (DyLight 550)

Catalog No. ABIN7354584

This anti-Integrin Alpha2b antibody is a Rabbit Polyclonal antibody detecting Integrin Alpha2b in FACS. Suitable for Human.

Quick Overview for Integrin Alpha2b antibody (AA 32-263) (DyLight 550) (ABIN7354584)

Target: Integrin Alpha2b (CD41)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Integrin Alpha2b antibody is conjugated to DyLight 550

Application: Flow Cytometry (FACS)

Product Details anti-Integrin Alpha2b Antibody (DyLight 550)

Binding Specificity: AA 32-263

Purpose: Anti-Human ITGA2B DyLight® 550 conjugated Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Human ITGA2B DyLight® 550 conjugated Antibody -Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human ITGA2B recombinant protein (Position: L32-D263).

Isotype: IgG

Application Details

Application Notes: Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells1. "Entrez Gene: ITGA2B integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41)". 2. Naik UP, Parise LV (1997). "Structure and function of platelet alpha IIb beta 3.". Curr. Opin. Hematol. 4(5): 317-22. 3. Quinn MJ, Byzova TV, Qin J et al. (2004). "Integrin alphaIIbbeta3 and its antagonism.". Arterioscler. Thromb. Vasc. Biol. 23 (6): 945-52.

Comment:

Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Target Details for Integrin Alpha2b

Target: Integrin Alpha2b (CD41)

Alternative Name: ITGA2B

Background:

Synonyms: Integrin alpha-IIb, GPalpha IIb, GPIIb, Platelet membrane glycoprotein IIb, CD41, Integrin alpha-IIb heavy chain, Integrin alpha-IIb light chain, form 1, Integrin alpha-IIb light chain, form 2, ITGA2B, GP2B, ITGAB

Tissue Specificity: Isoform 1 and isoform 2 are expressed in platelets and megakaryocytes, but not in reticulocytes. Not detected in Jurkat, nor in U937 cell lines (PubMed:2351656). Isoform 3 is expressed in prostate adenocarcinoma, as well as in several erythroleukemia, prostate adenocarcinoma and melanoma cell lines, including PC-3, DU-145, HEL, WM983A, WM983B and WM35. Not detected in platelets, nor in normal prostate (at protein level) (PubMed:9809974).

Background: Integrin alpha-IIb is a protein that in humans is encoded by the ITGA2B gene. It is mapped to 17q21.32. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. Alpha chain 2b undergoes post-translational cleavage to yield disulfide-linked light and heavy chains that join with beta 3 to form a fibrinogen receptor expressed in platelets that plays a crucial role in coagulation. Mutations that interfere with this role result in thrombasthenia. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.

Molecular Weight: 39 kDa

Gene ID: 3674

UniProt: P08514

Pathways: Integrin Complex