MIB1 antibody (AA 465-732)

Catalog No. ABIN6719398

This Rabbit Polyclonal antibody specifically detects MIB1 in ELISA and WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for MIB1 antibody (AA 465-732) (ABIN6719398)

Target: MIB1 (Mindbomb E3 Ubiquitin Protein Ligase 1 (MIB1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MIB1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details anti-MIB1 Antibody

Binding Specificity: AA 465-732

Purpose: Anti-Mib1/Mindbomb Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Mib1/Mindbomb Antibody Picoband® (ABIN6719398). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human Mib1 Mindbomb recombinant protein (Position: H465-E732).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Barsi, J. C., Rajendra, R., Wu, J. I., Artzt, K. Mind bomb1 is a ubiquitin ligase essential for mouse embryonic development and Notch signaling. Mech. Dev. 122: 1106-1117, 2005. 2. Henshall, D. C., Schindler, C. K., So, N. K., Lan, J.-Q., Meller, R., Simon, R. P.Death-associated protein kinase expression in human temporal lobe epilepsy. Ann. Neurol. 55: 485-494, 2004. 3. Jin, Y., Blue, E. K., Dixon, S., Shao, Z., Gallagher, P. J. A death-associated protein kinase (DAPK)-interacting protein, DIP-1, is an E3 ubiquitin ligase that promotes tumor necrosis factor-induced apoptosis and regulates the cellular levels of DAPK. J. Biol. Chem. 277: 46980-46986, 2002.

Comment:

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for MIB1

Target: MIB1 (Mindbomb E3 Ubiquitin Protein Ligase 1 (MIB1))

Alternative Name: MIB1

Background:

Synonyms: E3 ubiquitin-protein ligase MIB1, DAPK-interacting protein 1, DIP-1, Mind bomb homolog 1, RING-type E3 ubiquitin transferase MIB1 , Zinc finger ZZ type with ankyrin repeat domain protein 2, MIB1, DIP1, KIAA1323, ZZANK2

Tissue Specificity: Widely expressed at low level. Expressed at higher level in spinal cord, ovary, whole brain, and all specific brain regions examined.

Background: E3 ubiquitin-protein ligase MIB1 is an enzyme that in humans is encoded by the MIB1 gene. It is mapped to 18q11.2. This gene encodes a protein containing multiple ankyrin repeats and RING finger domains that functions as an E3 ubiquitin ligase. The encoded protein positively regulates Notch signaling by ubiquitinating the Notch receptors, thereby facilitating their endocytosis. This protein may also promote the ubiquitination and degradation of death-associated protein kinase 1 (DAPK1).

Molecular Weight: 110 kDa

Gene ID: 57534

Pathways: SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection