COMT antibody (AA 20-265)

Catalog No. ABIN6719465

The Rabbit Polyclonal anti-COMT antibody has been validated for WB and ELISA. It is suitable to detect COMT in samples from Rat and Mouse.

Quick Overview for COMT antibody (AA 20-265) (ABIN6719465)

Target: COMT (Catechol-O-Methyltransferase (COMT))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COMT antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-COMT Antibody

Binding Specificity: AA 20-265

Purpose: Anti-COMT Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-COMT Antibody Picoband® (ABIN6719465). Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived mouse COMT recombinant protein (Position: R20-S265).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Baker, K., Baldeweg, T., Sivagnanasundaram, S., Scambler, P., Skuse, D. COMT val108/158met modifies mismatch negativity and cognitive function in 22q11 deletion syndrome. Biol. Psychiat. 58: 23-31, 2005. 2. Lee, S.-G., Joo, Y., Kim, B., Chung, S., Kim, H.-L., Lee, I., Choi, B., Kim, C., Song, K.Association of Ala72Ser polymorphism with COMT enzyme activity and the risk of schizophrenia in Koreans. Hum. Genet. 116: 319-328, 2005.

Comment:

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for COMT

Target: COMT (Catechol-O-Methyltransferase (COMT))

Alternative Name: Comt

Background:

Synonyms: Catechol O-methyltransferase, Comt, Comt1

Background: Catechol O-methyltransferase, also called COMT, is one of the major mammalian enzymes involved in the metabolic degradation of catecholamines. This gene is mapped to 22q11.21. Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini.

Molecular Weight: 25 kDa, 29 kDa

Gene ID: 12846

UniProt: O88587

Pathways: Steroid Hormone Biosynthesis, SARS-CoV-2 Protein Interactome