CIC antibody (AA 1433-1527)

Catalog No. ABIN6719536

This Rabbit Polyclonal antibody specifically detects CIC in ELISA and IHC. It exhibits reactivity toward Mouse, Human and Rat.

Quick Overview for CIC antibody (AA 1433-1527) (ABIN6719536)

Target: CIC (Capicua Homolog (CIC))

Reactivity: Mouse, Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CIC antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Product Details anti-CIC Antibody

Binding Specificity: AA 1433-1527

Purpose: Anti-CIC Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CIC Antibody (ABIN6719536). Tested in ELISA, IHC applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human CIC recombinant protein (Position: E1433-Q1527).

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
ELISA, 0.1-0.5 μg/mL

Comment:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CIC

Target: CIC (Capicua Homolog (CIC))

Alternative Name: CIC

Background:

Synonyms: Protein capicua homolog, CIC, KIAA0306

Tissue Specificity: Expressed in fetal brain.

Background: Protein capicua homolog is a protein that in humans is encoded by the CIC gene. The protein encoded by this gene is an ortholog of the Drosophila melanogaster capicua gene, and is a member of the high mobility group (HMG)-box superfamily of transcriptional repressors. This protein contains a conserved HMG domain that is involved in DNA binding and nuclear localization, and a conserved C-terminus. Studies suggest that the N-terminal region of this protein interacts with Atxn1 (GeneID:6310), to form a transcription repressor complex, and in vitro studies suggest that polyglutamine-expansion of ATXN1 may alter the repressor activity of this complex. Mutations in this gene have been associated with olidogdendrogliomas (PMID:21817013). In addition, translocation events resulting in gene fusions of this gene with both DUX4 (GeneID:100288687) and FOXO4 (GeneID:4303) have been associated with round cell sarcomas. This gene is mapped to 19q13.2. Alternative splicing results in multiple transcript variants encoding different isoforms.

Molecular Weight: 55 kDa

Gene ID: 23152