HECTD3 antibody (C-Term)

Catalog No. ABIN6719595

The Rabbit Polyclonal anti-HECTD3 antibody (ABIN6719595) specifically detects HECTD3 in WB, IHC (fro), ICC, FACS and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for HECTD3 antibody (C-Term) (ABIN6719595)

Target: HECTD3 (HECT Domain Containing 3 (HECTD3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HECTD3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC)

Product Details anti-HECTD3 Antibody

Binding Specificity: C-Term

Purpose: Anti-HECTD3 Antibody

Sequence: HYASAKVCEE KLRYAAYNCV AIDTDMSPWE E

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: identical to the related mouse,rat sequences.

Characteristics: Anti-HECTD3 Antibody. Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human HECTD3, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat ELISA, 0.1-0.5 μg/mL, Human

Comment:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for HECTD3

Target: HECTD3 (HECT Domain Containing 3 (HECTD3))

Alternative Name: HECTD3

Background:

Background: The protein encoded by this gene transfers ubiquitin from an E2 ubiquitin-conjugating enzyme to targeted substrates, leading to the degradation of those substrates. This gene is mapped to 1p34.1. The encoded protein has been shown to transfer ubiquitin to TRIOBP to facilitate cell cycle progression, and to STX8.

Gene Full Name: HECT domain E3 ubiquitin protein ligase 3

Molecular Weight: 97 kDa

Gene ID: 79654

UniProt: Q5T447