NONO antibody (N-Term)

Catalog No. ABIN6719600

The Mouse Monoclonal anti-NONO antibody has been validated for WB, IF, ICC and FACS. It is suitable to detect NONO in samples from Human.

Quick Overview for NONO antibody (N-Term) (ABIN6719600)

Target: NONO (Non-POU Domain Containing, Octamer-Binding (NONO))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NONO antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 11E2

Product Details anti-NONO Antibody

Binding Specificity: AA 1-35, N-Term

Purpose: Anti-nmt55 p54nrb NONO Antibody (monoclonal, 11E2)

Sequence: MQSNKTFNLE KQNHTPRKHH QHHHQQQHHQ QQQQQ

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-nmt55 p54nrb NONO Antibody (monoclonal, 11E2) (ABIN6719600). Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human nmt55 p54nrb, identical to the related mouse and rat sequences.

Isotype: IgG1

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunocytochemistry, 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 5 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells

Comment:

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for NONO

Target: NONO (Non-POU Domain Containing, Octamer-Binding (NONO))

Alternative Name: NONO

Background:

Synonyms: Non-POU domain-containing octamer-binding protein, NonO protein, 54 kDa nuclear RNA- and DNA-binding protein, 55 kDa nuclear protein, DNA-binding p52/p100 complex, 52 kDa subunit, NMT55, p54 (nrb), p54nrb, NONO, NRB54

Tissue Specificity: Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Also found in a number of breast tumor cell lines.

Background: Non-POU domain-containing octamer-binding protein is a protein that in humans is encoded by the NONO gene. This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16.

Molecular Weight: 60 kDa

Gene ID: 4841

UniProt: Q15233