CCL6 antibody (AA 22-116)

Catalog No. ABIN6719644

This Rabbit Polyclonal antibody specifically detects CCL6 in WB, IHC and ELISA. It exhibits reactivity toward Mouse and Rat.

Quick Overview for CCL6 antibody (AA 22-116) (ABIN6719644)

Target: CCL6 (Chemokine (C-C Motif) Ligand 6 (CCL6))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-CCL6 Antibody

Binding Specificity: AA 22-116

Purpose: Anti-CCL6 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CCL6 Antibody Picoband® (ABIN6719644). Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived mouse CCL6 recombinant protein (Position: G22-A116).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
ELISA (Cap), 1-5 μg/mL
1. Orlofsky et al. Novel expression pattern of a new member of the MIP-1 family of cytokine-like genes. Cell Regul. 2 (5), 403-412 (1991). 2. Ma et al. The C10/CCL6 chemokine and CCR1 play critical roles in the pathogenesis of IL-13-induced inflammation and remodeling. J Immunol. 2004 Feb 1,172(3):1872-81.

Comment:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CCL6

Target: CCL6 (Chemokine (C-C Motif) Ligand 6 (CCL6))

Alternative Name: Ccl6

Background:

Synonyms: C-C motif chemokine 6, Protein C10, Small-inducible cytokine A6, CCL6 (22-95), CCL6 (23-95), Ccl6, C10, Scya6

Tissue Specificity: Expressed in myelopoietic bone marrow cultures stimulated by GM-CSF.

Background: Chemokine (C-C motif) ligand 6 (CCL6) is a small cytokine belonging to the CC chemokine family that has only been identified in rodents. In mice, CCL6 is expressed in cells from neutrophil and macrophage lineages, and can be greatly induced under conditions suitable for myeloid cell differentiation. It is highly expressed in bone marrow cultures that have been stimulated with the cytokine GM-CSF. Some low levels of gene expression also occur in certain cell lines of myeloid origin (e.g. the immature myeloid cell lines DA3 and 32D cl3, and the macrophage cell line P388D) that can also be greatly induced in culture with GM-CSF. However, in activated T cell lines, expression of CCL6 is greatly reduced.CCL6 can also be induced in the mouse lung by the cytokine interleukin 13. Mouse CCL6 is located on chromosome 11. The cell surface receptor for CCL6 is believed to be the chemokine receptor CCR1.

Molecular Weight: 11 kDa

Gene ID: 20305

UniProt: P27784

Pathways: Response to Water Deprivation, Cell-Cell Junction Organization