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Mitochondrially Encoded NADH Dehydrogenase 4 (MT-ND4) (AA 10-459) antibody Primary Antibody

MT-ND4 Reactivity: Human ELISA, WB Host: Rabbit Polyclonal
Catalog No. ABIN6719648
$240.00
Plus shipping costs $45.00
100 μg
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  • Target
    Binding Specificity
    AA 10-459
    Reactivity
    Human
    Host
    Rabbit
    Clonality
    Polyclonal
    Application
    ELISA, Western Blotting (WB)
    Purpose
    Rabbit IgG polyclonal antibody for MT-ND4 detection. Tested with WB, Direct ELISA in Human.
    Cross-Reactivity (Details)
    No cross reactivity with other proteins.
    Characteristics
    Rabbit IgG polyclonal antibody for MT-ND4 detection. Tested with WB, Direct ELISA in Human.
    Purification
    Immunogen affinity purified.
    Immunogen
    E.coli-derived human MT-ND4 recombinant protein (Position: M10-S459).
    Isotype
    IgG
  • Target
    Alternative Name
    MT-ND4 (MT-ND4 Antibody Abstract)
    Background

    Synonyms: NADH-ubiquinone oxidoreductase chain 4, NADH dehydrogenase subunit 4, MT-ND4, MTND4, NADH4, ND4,

    Background: NADH-ubiquinone oxidoreductase chain 4 is a protein that in humans is encoded by the mitochondrial geneMT-ND4. MT-ND4 is a subunit of the respiratory chain Complex I that is believed to belong to the minimal assembly of core proteins required to catalyze NADH dehydrogenation and electron transfer to ubiquinone (coenzyme Q10). Initially, NADH binds to Complex I and transfers two electrons to the isoalloxazine ring of the flavin mononucleotide (FMN) prosthetic arm to form FMNH2. The electrons are transferred through a series of iron-sulfur (Fe-S) clusters in the prosthetic arm and finally to coenzyme Q10 (CoQ), which is reduced to ubiquinol (CoQH2). The flow of electrons changes the redox state of the protein, resulting in a conformational change and pK shift of the ionizable side chain, which pumps four hydrogen ions out of the mitochondrial matrix.

    Gene ID
    4538
    UniProt
    P03905
  • Application Notes

    Application details: Western blot|0.1-0.5&mu,g/mL Direct ELISA|0.1-0.5&mu,g/mL

    Comment

    Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Buffer
    Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    At -20°C for one year. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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