Mitochondrially Encoded NADH Dehydrogenase 4 (MT-ND4) (AA 10-459) antibody (HRP)

Catalog No. ABIN8010801

The Rabbit Polyclonal anti-Mitochondrially Encoded NADH Dehydrogenase 4 antibody is suitable to detect Mitochondrially Encoded NADH Dehydrogenase 4 in samples from Human. It has been validated for WB, ELISA and IHC.

Quick Overview for Mitochondrially Encoded NADH Dehydrogenase 4 (MT-ND4) (AA 10-459) antibody (HRP) (ABIN8010801)

Target: Mitochondrially Encoded NADH Dehydrogenase 4 (MT-ND4)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details

Binding Specificity: AA 10-459

Purpose: Anti-MT-ND4 Antibody HRP Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MT-ND4 recombinant protein (Position: M10-S459).

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details

Target: Mitochondrially Encoded NADH Dehydrogenase 4 (MT-ND4)

Alternative Name: MT-ND4

Background:

Background: NADH-ubiquinone oxidoreductase chain 4 is a protein that in humans is encoded by the mitochondrial gene MT-ND4. MT-ND4 is a subunit of the respiratory chain Complex I that is believed to belong to the minimal assembly of core proteins required to catalyze NADH dehydrogenation and electron transfer to ubiquinone (coenzyme Q10). Initially, NADH binds to Complex I and transfers two electrons to the isoalloxazine ring of the flavin mononucleotide (FMN) prosthetic arm to form FMNH2. The electrons are transferred through a series of iron-sulfur (Fe-S) clusters in the prosthetic arm and finally to coenzyme Q10 (CoQ), which is reduced to ubiquinol (CoQH2). The flow of electrons changes the redox state of the protein, resulting in a conformational change and pK shift of the ionizable side chain, which pumps four hydrogen ions out of the mitochondrial matrix.

Gene Full Name: NADH dehydrogenase subunit 4

Gene ID: 4538

UniProt: P03905