Biotin antibody (DyLight 405)

Catalog No. ABIN6720658

Supplier Product No.: 600-446-098

This Rabbit Polyclonal antibody specifically detects Biotin in WB, ELISA, FM and FLISA.

Quick Overview for Biotin antibody (DyLight 405) (ABIN6720658)

Target: Biotin

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Biotin antibody is conjugated to DyLight 405

Application: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM), FLISA

Product Details anti-Biotin Antibody (DyLight 405)

Supplier Product No.: 600-446-098

Supplier: Rockland

Purpose: Biotin Antibody Dylight™ 405 Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Biotin conjugated IgG and Biotin conjugated Bovine Serum Albumin.

Purification: Anti-Biotin antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads.

Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)

Isotype: IgG

Labeling Ratio: 3.0

Application Details

Application Notes:

FLISA_Dilution: >1:20,000

ELISA_Dilution: 1:10,000 - 1:50,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: 1:10,000-1:25,000

Comment:

The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store Anti-Biotin antibody at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Biotin

Target: Biotin

Target Type: Chemical

Background:

Synonyms: rabbit anti-Biotin Antibody Dylight™ 405 Conjugated, rabbit anti Biotin Antibody Dylight™ 405 Conjugated

Background: Anti-Biotin antibody conjugated to DyLight 405 is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.