V5 Epitope Tag antibody (AA 95-108) (DyLight 549)

Catalog No. ABIN6720676

Supplier Product No.: 600-442-378

This Rabbit Polyclonal antibody specifically detects V5 Epitope Tag in WB, FM and FLISA. It has been mentioned in 6+ publications

Quick Overview for V5 Epitope Tag antibody (AA 95-108) (DyLight 549) (ABIN6720676)

Target: V5 Epitope Tag

Host: Rabbit

Clonality: Polyclonal

Conjugate: This V5 Epitope Tag antibody is conjugated to DyLight 549

Application: Western Blotting (WB), Fluorescence Microscopy (FM), FLISA

Product Details anti-V5 Epitope Tag Antibody (DyLight 549)

Binding Specificity: AA 95-108

Supplier Product No.: 600-442-378

Supplier: Rockland

Purpose: V5 Epitope Tag Antibody Dylight™ 549 Conjugated

Cross-Reactivity (Details): The antibody recognizes the V5 epitope tag (Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

Purification: This affinity purified antibody is directed against the V5 epitope tag and is useful in determining its presence in over expressed proteins in various assays.

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 95-108 (G-K-P-I-P-N-P-L-L-G-L-D-S-T) of the V protein conjugated to KLH using maleimide.

Isotype: IgG

Labeling Ratio: 3.6

Biological Activity Comment: 5.1

Application Details

Application Notes:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: 1:10,000 - 1:25,000

Other: User Optimized

Comment:

Suggested Applications: EM, IF, IHC, Multiplex
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

References for anti-V5 Epitope Tag antibody (DyLight 549) (ABIN6720676)

Brovero, Fortier, Hu, Lovejoy, Newell, Palmateer, Tzeng, Lee, Zinn, Arbeitman: "Investigation of Drosophila fruitless neurons that express Dpr/DIP cell adhesion molecules." in: eLife, Vol. 10, (2022) (PubMed).

Mark, Lai, Zarin, Manning, Pollington, Litwin-Kumar, Cardona, Truman, Doe: "A developmental framework linking neurogenesis and circuit formation in the Drosophila CNS." in: eLife, Vol. 10, (2021) (PubMed).

Fukuda, Narendran, Varshney, Nagasaka, Wang, Ambati, Apicella, Pereira, Fowler, Yasuma, Hirahara, Yasuma, Huang, Yerramothu, Makin, Wang, Baker, Marion, Huang, Baghdasaryan, Ambati, Ambati, Banerjee, Bonilha, Tolstonog, Held, Ogura, Terasaki, Oshika, Bhat: "Alu complementary DNA is enriched in atrophic macular degeneration and triggers retinal pigmented epithelium toxicity via cytosolic innate immunity." in: Science advances, Vol. 7, Issue 40, pp. eabj3658, (2021) (PubMed).

Sancer, Kind, Plazaola-Sasieta, Balke, Pham, Hasan, Münch, Courgeon, Mathejczyk, Wernet: "Modality-Specific Circuits for Skylight Orientation in the Fly Visual System." in: Current biology : CB, Vol. 29, Issue 17, pp. 2812-2825.e4, (2020) (PubMed).

Sen, Chanchani, Southall, Doe: "Neuroblast-specific open chromatin allows the temporal transcription factor, Hunchback, to bind neuroblast-specific loci." in: eLife, Vol. 8, (2019) (PubMed).

Manning, Doe: "Immunofluorescent antibody staining of intact Drosophila larvae." in: Nature protocols, Vol. 12, Issue 1, pp. 1-14, (2017) (PubMed).

Target Details for V5 Epitope Tag

Target: V5 Epitope Tag

Target Type: Tag

Background:

Synonyms: Rabbit Anti-V5 Epitope Tag DyLight 549™ Conjugated Antibody, Rabbit Anti V5 Epitope Tag Antibody DyLight 549™ Conjugation, Rabbit Anti-V5 Tag DyLight 549™ Conjugated Antibody

Background: In order to improve expression levels, solubility, folding, purification and detection of recombinant proteins, a very common strategy is the fusion of peptides or proteins also known as ""tags"", to the target protein. Because these tags are entities with known sequences and well characterized physicochemical properties, they are an essential tool in molecular biology that facilitates expression and purification of recombinant proteins. Because fusion tags constitute themselves antigenic epitopes for which antibodies can be developed they particularly useful for specific detection of the target protein. This Anti-V5 Epitope Tag Antibody generated in rabbit is conjugated to DyLight™549.