CD4 antibody (AA 231-330)

Catalog No. ABIN672936

This anti-CD4 antibody is a Rabbit Polyclonal antibody detecting CD4 in FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc) and IF (p). Suitable for Human, Mouse and Rat. This Primary Antibody has been cited in 8+ publications.

Quick Overview for CD4 antibody (AA 231-330) (ABIN672936)

Target: CD4

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-CD4 Antibody

Binding Specificity: AA 231-330

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Hamster

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse CD4

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-CD4 antibody (ABIN672936)

Wang, Johnson, He, Rong, Wong, Nigam, Behfar, Xu, Christman: "Humanized mouse model for assessing the human immune response to xenogeneic and allogeneic decellularized biomaterials." in: Biomaterials, Vol. 129, pp. 98-110, (2017) (PubMed).

Willett, Liu, Berke, Giannini, Schmid, Jia, Wang, Wang, Samson, Yu, Wang, Nawshad, Reinhardt: "Standardized Rat Model Testing Effects of Inflammation and Grafting on Extraction Healing." in: Journal of periodontology, Vol. 88, Issue 8, pp. 799-807, (2017) (PubMed).

Matsubara, Matsubara, Mori, Uchikura, Hamada, Fujioka, Hashimoto, Matsumoto: "Immune activation during the implantation phase causes preeclampsia-like symptoms via the CD40-CD40 ligand pathway in pregnant mice." in: Hypertension research : official journal of the Japanese Society of Hypertension, Vol. 39, Issue 6, pp. 407-14, (2017) (PubMed).

Ju, Xing, Yang, Zheng, Jia, Zhang, Ren: "An effective cytokine adjuvant vaccine induces autologous T-cell response against colon cancer in an animal model." in: BMC immunology, Vol. 17, Issue 1, pp. 31, (2016) (PubMed).

Tzeng, Wu, Peng, Cheng, Tang, Huang, Chu: "Hypercapnic acidosis prolongs survival of skin allografts." in: The Journal of surgical research, (2015) (PubMed).

Cheng, Guo, Qiao, Liu, Nie, Li, Wang, Jiang: "T cell immunohistochemistry refines lung transplant acute rejection diagnosis and grading." in: Diagnostic pathology, Vol. 8, pp. 168, (2013) (PubMed).

Zhang, Fei, Gao, Liu, Zhang: "The role of CXCR3 in the induction of primary biliary cirrhosis." in: Clinical & developmental immunology, Vol. 2011, pp. 564062, (2011) (PubMed).

Target Details for CD4

Target: CD4

Alternative Name: CD4

Background:

Synonyms: L3T4, Ly-4, T-cell surface glycoprotein CD4, T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3, CD4

Background: Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation.

Gene ID: 12504

UniProt: P06332

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events