Recombinant Nucleolin antibody (Sortase,His tag,AVI tag)

Catalog No. ABIN6731026

The Mouse Monoclonal anti-Nucleolin antibody is suitable to detect Nucleolin in samples from Human and Dog. It has been validated for WB.

Quick Overview for Recombinant Nucleolin antibody (Sortase,His tag,AVI tag) (ABIN6731026)

Target: Nucleolin (NCL)

Antibody Type: Recombinant Antibody

Reactivity: Human, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nucleolin antibody is conjugated to Sortase,His tag,AVI tag

Application: Western Blotting (WB)

Product Details anti-Nucleolin Antibody (Sortase,His tag,AVI tag)

Purpose: AbFlex Nucleolin antibody (rAb)

Brand: AbFlex®

Characteristics: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports.

Purification: Protein A Chromatography

Immunogen: This antibody was raised against a salt-extracted nuclear matrix preparation from MDCK (canine kidney) cells.

Isotype: IgG2a

Application Details

Application Notes: WB*: 0.1 - 1 μg/mL *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, see Catalog No. 39541.

Comment:

This recombinant antibody has been generated using defined DNA sequences to achieve high specificity and high reproducibility. The unique advantages of this antibody are its flexible labeling and purification options as it contains a sortase recognition motif (LPXTG) to covalently add fluorophores, enzymatic substrates (HRP, AP...etc), peptides, DNA, drugs or other labels to the antibody in a directed and reproducible manner. Every antibody also contains a 6xHis Tag, which can be used with nickel-based purification systems, and a Biotinylation Tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for Nucleolin

Target: Nucleolin (NCL)

Alternative Name: Nucleolin

Background: AbFlex Nucleolin antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. Nucleolin (NCL, C23) is an abundant and ubiquitously expressed protein that is a major component of the nucleolus. Nucleolin seems to be overexpressed in highly proliferative cells and is involved in many aspects of gene expression: chromatin remodeling, DNA recombination and replication, RNA transcription by RNA polymerase I and II (RNA pol II), rRNA processing, mRNA stabilization, cytokinesis and apoptosis. Its role as a histone chaperone has recently been identified. It is involved in the synthesis and maturation of ribosomes, and is located mainly in dense fibrillar regions of the nucleolus.

Molecular Weight: 100 kDa

NCBI Accession: XP_850477

Pathways: Ribonucleoprotein Complex Subunit Organization