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POLB antibody

This anti-POLB antibody is a Mouse Monoclonal antibody detecting POLB in WB and ELISA. Suitable for Human, Mouse, Rat, Hamster and Frog.
Catalog No. ABIN6735601

Quick Overview for POLB antibody (ABIN6735601)

Target

See all POLB Antibodies
POLB (Polymerase (DNA Directed), beta (POLB))

Reactivity

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  • 1
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  • 1
Human, Mouse, Rat, Hamster, Frog

Host

  • 57
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Mouse

Clonality

  • 50
  • 9
Monoclonal

Conjugate

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  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This POLB antibody is un-conjugated

Application

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Western Blotting (WB), ELISA
  • Specificity

    Recognizes rat DNA Polymerase, beta. Species cross-reactivity: Mouse, human, hamster and frog.

    Purification

    Protein A/G purified

    Immunogen

    Purified Rat DNA Pol beta (~39kD).

    Isotype

    IgG
  • Application Notes

    Approved: ELISA (1:10000 - 1:50000), WB (1 - 5 μg/mL)

    Usage: Suitable for use in ELISA and Western Blot. Western Blot: 1-5 μg/mL. The antibody has detected ~39kD band from HeLa and other cells and tissues. ELISA: 1:10000-1:50000 using 50-100 ng control peptide/well.

    Comment

    Target Species of Antibody: Rat

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    100 mM Tris,  pH 7.5, 0.2 % BSA, 0.5 % sodium azide, 40 % glycerol.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeat freeze-thaw cycles.

    Storage

    4 °C,-20 °C

    Storage Comment

    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • Target

    POLB (Polymerase (DNA Directed), beta (POLB))

    Alternative Name

    POLB / DNA Polymerase Beta

    Background

    Name/Gene ID: POLB

    Synonyms: POLB, DNA pol beta, Mutant DNA polymerase beta, DNA polymerase beta, DNA polymerase beta subunit

    Gene ID

    5423

    UniProt

    P06746

    Pathways

    DNA Damage Repair
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