BRD2 antibody (AA 524-573)

Catalog No. ABIN214728

Product Details anti-BRD2 Antibody

Target: BRD2 (Bromodomain Containing 2 (BRD2))

Binding Specificity: AA 524-573

Reactivity: Human, Mouse, Cow, Dog, Rat, Horse, Pig, Hamster, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRD2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human BRD2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Pig (100%) Xenopus (92%) Zebrafish (91%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa524-573 of human BRD2 (P25440, NP_005095). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Horse, Pig, Opossum (100%), Xenopus (92%), Zebrafish (91%), Guinea pig (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for BRD2

Target: BRD2 (Bromodomain Containing 2 (BRD2))

Alternative Name: BRD2 / RING3 (BRD2 Products)

Synonyms: D6S113E antibody, FSH antibody, FSRG1 antibody, NAT antibody, RING3 antibody, RNF3 antibody, AW228947 antibody, D17H6S113E antibody, Frg-1 antibody, Fsrg-1 antibody, Fsrg1 antibody, Nat antibody, Ring3 antibody, Rnf3 antibody, mKIAA4005 antibody, brd2-a antibody, fsh antibody, fsrg1 antibody, nat antibody, ring3 antibody, rnf3 antibody, brd2 antibody, fe47f02 antibody, fi85h05 antibody, wu:fe47f02 antibody, wu:fi85h05 antibody, Ring3 protein antibody, bromodomain containing 2 antibody, bromodomain containing 2 S homeolog antibody, bromodomain containing 2a antibody, CHLREDRAFT_190116 antibody, CHLREDRAFT_150233 antibody, CHLREDRAFT_182576 antibody, CHLREDRAFT_188250 antibody, BRD2 antibody, Brd2 antibody, brd2.S antibody, brd2a antibody

Background: Name/Gene ID: BRD2
Subfamily: BRD
Family: Protein Kinase

Synonyms: BRD2, Bromodomain containing 2, FSRG1, KIAA9001, NaT, RING3, RNF3, O27.1.1, Bromodomain-containing 2, D6S113E, FSH

Gene ID: 6046

NCBI Accession: NP_005095

UniProt: P25440

Pathways: Chromatin Binding, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection