FOXA1 antibody (AA 395-444)

Catalog No. ABIN6736377

The Rabbit Polyclonal anti-FOXA1 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect FOXA1 in samples from Human, Rat, Mouse, Dog, Cow, Pig, Zebrafish (Danio rerio), Guinea Pig and Monkey.

Quick Overview for FOXA1 antibody (AA 395-444) (ABIN6736377)

Target: FOXA1 (Forkhead Box A1 (FOXA1))

Reactivity: Human, Rat, Mouse, Dog, Cow, Pig, Zebrafish (Danio rerio), Guinea Pig, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-FOXA1 Antibody

Binding Specificity: AA 395-444

Brand: IHC-plus™

Specificity: Human FOXA1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Pig, Guinea pig (100%) Zebrafish (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa395-444 of human FOXA1 (NP_004487). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Galago, Mouse, Rat, Elephant, Panda, Dog, Bovine, Pig, Opossum, Guinea pig, Lizard (100%), Xenopus (93%), Bat (92%), Stickleback, Zebrafish (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for FOXA1

Target: FOXA1 (Forkhead Box A1 (FOXA1))

Alternative Name: FOXA1

Background: Name/Gene ID: FOXA1
Family: Transcription factor

Synonyms: FOXA1, Forkhead box A1, Forkhead box protein A1, HNF-3-alpha, HNF-3A, TCF-3A, TCF3A, HNF3A, Transcription factor 3A

Gene ID: 3169

NCBI Accession: NP_004487

UniProt: P55317

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis