PHKG2 antibody (N-Term)

Catalog No. ABIN6736422

The Rabbit Polyclonal anti-PHKG2 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect PHKG2 in samples from Human, Dog, Cow, Goat, Guinea Pig, Rabbit, Sheep, Bat, Monkey and Pig.

Quick Overview for PHKG2 antibody (N-Term) (ABIN6736422)

Target: PHKG2 (phosphorylase Kinase, gamma 2 (Testis) (PHKG2))

Reactivity: Human, Dog, Cow, Goat, Guinea Pig, Rabbit, Sheep, Bat, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHKG2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PHKG2 Antibody

Binding Specificity: N-Term

Brand: IHC-plus™

Specificity: Human PHKG2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Sheep, Dog, Bovine, Goat, Rabbit, Pig, Guinea pig (100%) Mouse, Rat, Zebrafish (92%) Xenopus (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human PHKG2 (P15735, NP_000285). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Marmoset, Sheep, Goat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Pig, Opossum, Guinea pig (100%), Mouse, Rat, Platypus, Xenopus, Seabass, Salmon, Stickleback, Zebrafish (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PHKG2

Target: PHKG2 (phosphorylase Kinase, gamma 2 (Testis) (PHKG2))

Alternative Name: PHKG2

Background: Name/Gene ID: PHKG2
Subfamily: PHK
Family: Protein Kinase

Synonyms: PHKG2, GSD9C, Phosphorylase kinase, gamma 2, PHK-gamma-T, PSK-C3

Gene ID: 5261

NCBI Accession: NP_000285

UniProt: P15735

Pathways: Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process