E2F2 antibody (AA 191-240)

Catalog No. ABIN6736712

The Rabbit Polyclonal anti-E2F2 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect E2F2 in samples from Human, Mouse, Rabbit, Dog, Cow, Horse, Monkey and Bat.

Quick Overview for E2F2 antibody (AA 191-240) (ABIN6736712)

Target: E2F2 (E2F Transcription Factor 2 (E2F2))

Reactivity: Human, Mouse, Rabbit, Dog, Cow, Horse, Monkey, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-E2F2 Antibody

Binding Specificity: AA 191-240

Brand: IHC-plus™

Specificity: Human E2F2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Bovine, Horse (100%) Guinea pig (92%) Mouse, Rabbit (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa191-240 of human E2F2 (Q14209, NP_004082). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Panda, Dog, Bovine, Bat, Horse (100%), Gorilla, Galago, Elephant, Guinea pig (92%), Mouse, Rabbit (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for E2F2

Target: E2F2 (E2F Transcription Factor 2 (E2F2))

Alternative Name: E2F2

Background: Name/Gene ID: E2F2

Synonyms: E2F2, E2F-2, E2F transcription factor 2, Transcription factor E2F2

Gene ID: 1870

NCBI Accession: NP_004082

UniProt: Q14209

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication