Fibrillin 1 antibody (N-Term)

Catalog No. ABIN6736719

Product Details anti-Fibrillin 1 Antibody

Target: Fibrillin 1 (FBN1)

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Fibrillin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human FBN1 / Fibrillin 1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Horse (100%) Bovine, Pig (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human FBN1 (Q75N89). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Galago, Rat, Panda, Horse (100%), Marmoset, Elephant, Bovine, Pig (92%), Guinea pig (85%), Mouse (78%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Fibrillin 1

Target: Fibrillin 1 (FBN1)

Alternative Name: FBN1 / Fibrillin 1 (FBN1 Products)

Background: Name/Gene ID: FBN1

Synonyms: FBN1, ACMICD, Fibrillin 1, Fibrillin-1, FBN, ECTOL1, GPHYSD2, MASS, MFS1, OCTD, SSKS, WMS2, Fibrillin 1 (Marfan syndrome), Fibrillin 15, SGS, WMS

Gene ID: 2200

Pathways: Maintenance of Protein Location, SARS-CoV-2 Protein Interactome