NIFK antibody (AA 140-189)

Catalog No. ABIN6736736

The Rabbit Polyclonal anti-NIFK antibody has been validated for WB, IHC and IHC (p). It is suitable to detect NIFK in samples from Human, Horse and Pig.

Quick Overview for NIFK antibody (AA 140-189) (ABIN6736736)

Target: NIFK (MKI67IP) (MKI67 FHA Domain-Interacting Nucleolar Phosphoprotein (MKI67IP))

Reactivity: Human, Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NIFK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NIFK Antibody

Binding Specificity: AA 140-189

Brand: IHC-plus™

Specificity: Human MKI67IP

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Dog, Rabbit (92%) Horse (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa140-189 of human MKI67IP (Q9BYG3, NP_115766). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Marmoset, Rat, Panda, Dog, Rabbit (92%), Mouse, Zebra finch (91%), Galago, Hamster, Elephant, Bovine, Horse (85%), Bat (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for NIFK

Target: NIFK (MKI67IP) (MKI67 FHA Domain-Interacting Nucleolar Phosphoprotein (MKI67IP))

Alternative Name: NIFK / MKI67IP

Background: Name/Gene ID: NIFK

Synonyms: NIFK, HNIFK, MKI67IP, Nopp34

Gene ID: 84365

NCBI Accession: NP_115766

UniProt: Q9BYG3