NIFK antibody

Catalog No. ABIN7225410

This Rabbit Polyclonal antibody specifically detects NIFK in WB, ELISA, IHC and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for NIFK antibody (ABIN7225410)

Target: NIFK (MKI67IP) (MKI67 FHA Domain-Interacting Nucleolar Phosphoprotein (MKI67IP))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NIFK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-NIFK Antibody

Purpose: Rabbit Anti-NIFK Polyclonal Antibody

Specificity: NIFK Polyclonal Antibody detects endogenous levels of NIFK protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human NIFK around the non-phosphorylation site of T234

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:5000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for NIFK

Target: NIFK (MKI67IP) (MKI67 FHA Domain-Interacting Nucleolar Phosphoprotein (MKI67IP))

Alternative Name: NIFK

Background: MKI67IP, NIFK, NOPP34, MKI67 FHA domain-interacting nucleolar phosphoprotein, Nucleolar phosphoprotein Nopp34, Nucleolar protein interacting with the FHA domain of pKI-67, hNIFKNIFK (nucleolar protein interacting with the FHA domain of MKI67) encodes a protein that interacts with the forkhead-associated domain of the Ki-67 antigen. The encoded protein may bind RNA and may play a role in mitosis and cell cycle progression. Multiple pseudogenes exist on chromosomes 5, 10, 12, 15, and 19.

Molecular Weight: 34kD

Gene ID: 84365

UniProt: Q9BYG3