NIFK antibody (pThr234)

Catalog No. ABIN7225408

The Rabbit Polyclonal anti-NIFK antibody (ABIN7225408) specifically detects NIFK in ELISA, IHC and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for NIFK antibody (pThr234) (ABIN7225408)

Target: NIFK (MKI67IP) (MKI67 FHA Domain-Interacting Nucleolar Phosphoprotein (MKI67IP))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NIFK antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-NIFK Antibody

Binding Specificity: pThr234

Purpose: Rabbit Anti-NIFK (phospho Thr234) Polyclonal Antibody

Specificity: Phospho-NIFK (T234) Polyclonal Antibody detects endogenous levels of NIFK protein only when phosphorylated at T234.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human NIFK Phospho-Thr234

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:40000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for NIFK

Target: NIFK (MKI67IP) (MKI67 FHA Domain-Interacting Nucleolar Phosphoprotein (MKI67IP))

Alternative Name: NIFK

Background: MKI67IP, NIFK, NOPP34, MKI67 FHA domain-interacting nucleolar phosphoprotein, Nucleolar phosphoprotein Nopp34, Nucleolar protein interacting with the FHA domain of pKI-67, hNIFKNIFK (nucleolar protein interacting with the FHA domain of MKI67) encodes a protein that interacts with the forkhead-associated domain of the Ki-67 antigen. The encoded protein may bind RNA and may play a role in mitosis and cell cycle progression. Multiple pseudogenes exist on chromosomes 5, 10, 12, 15, and 19.

Gene ID: 84365

UniProt: Q9BYG3