NCOR2 antibody (AA 511-560)

Catalog No. ABIN6736738

This Rabbit Polyclonal antibody specifically detects NCOR2 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Pig and Zebrafish (Danio rerio).

Quick Overview for NCOR2 antibody (AA 511-560) (ABIN6736738)

Target: NCOR2 (Nuclear Receptor Co-Repressor 2 (NCOR2))

Reactivity: Human, Mouse, Rat, Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCOR2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NCOR2 Antibody

Binding Specificity: AA 511-560

Brand: IHC-plus™

Specificity: Human NCOR2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Pig (100%) Bovine (92%) Mouse, Rat (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa511-560 of human NCOR2 (Q9Y618, NP_006303). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Pig, Trout (100%), Marmoset, Bovine, Turkey (92%), Mouse, Rat, Panda, Zebra finch, Chicken, Xenopus, Slime mold (85%), Drosophila (84%), Galago (81%), Bat, Zebrafish, Sordaria (80%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for NCOR2

Target: NCOR2 (Nuclear Receptor Co-Repressor 2 (NCOR2))

Alternative Name: NCOR2 / SMRT

Background: Name/Gene ID: NCOR2

Synonyms: NCOR2, CTG26, Nuclear receptor corepressor 2, SMAP270, SMRT, SMRTE, TRAC, N-CoR2, TNRC14, TRAC-1, TRAC1, CTG repeat protein 26, SMRTE-tau, T3 receptor-associating factor

Gene ID: 9612

NCBI Accession: NP_006303

UniProt: Q9Y618

Pathways: Notch Signaling, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha