Paired Box 3 antibody (AA 400-449)

Catalog No. ABIN214133

The Rabbit Polyclonal anti-Paired Box 3 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect Paired Box 3 in samples from Human, Mouse, Rat, Dog, Cow, Horse, Guinea Pig, Rabbit, Zebrafish (Danio rerio), Bat, Monkey, Chicken and Xenopus laevis.

Quick Overview for Paired Box 3 antibody (AA 400-449) (ABIN214133)

Target: Paired Box 3 (PAX3)

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Guinea Pig, Rabbit, Zebrafish (Danio rerio), Bat, Monkey, Chicken, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Paired Box 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Paired Box 3 Antibody

Binding Specificity: AA 400-449

Brand: IHC-plus™

Specificity: Human PAX3

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Guinea pig, Chicken, Xenopus (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa400-449 of human PAX3 (P23760, NP_852122). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus (100%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Paired Box 3

Target: Paired Box 3 (PAX3)

Alternative Name: PAX3

Background: Name/Gene ID: PAX3

Synonyms: PAX3, CDHS, Paired domain gene 3, Paired box protein Pax-3, Paired domain gene HuP2, Waardenburg syndrome 1, WS1, HUP2, Paired box 3, Paired box homeotic gene 3, WS3

Gene ID: 5077

NCBI Accession: NP_852122

Pathways: Sensory Perception of Sound, Tube Formation