His Tag antibody (Alkaline Phosphatase (AP))

Catalog No. ABIN6737086

This Mouse Monoclonal antibody specifically detects His Tag in WB and ELISA. It exhibits reactivity toward .

Quick Overview for His Tag antibody (Alkaline Phosphatase (AP)) (ABIN6737086)

Target: His Tag

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This His Tag antibody is conjugated to Alkaline Phosphatase (AP)

Application: Western Blotting (WB), ELISA

Product Details anti-His Tag Antibody (Alkaline Phosphatase (AP))

Sequence: HHHHHH

Specificity: Recognizes native and denatured-reduced forms of synthetic poly-histidine or poly-histidine-tagged fusion proteins. Recognizes fusion proteins expressed by prokaryotic pET, pRSET and pTrc expression vectors.

Purification: Affinity purified

Immunogen: His-tag-fusion protein.

Type of Immunogen: Fusion protein

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA (1:10000 - 1:50000), WB (1:1000 - 1:5000)

Usage: Suitable for use in ELISA and Western Blot. Western Blot: 1:1000-1:5000. ELISA: 1:10000-1:50000. Use 50-100 ng control antigen/well. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months. APC is light sensitive.

Target Details for His Tag

Target: His Tag

Target Type: Tag