TLE2 antibody (AA 201-250)

Catalog No. ABIN6737675

The Rabbit Polyclonal anti-TLE2 antibody has been validated for WB. It is suitable to detect TLE2 in samples from Human, Mouse, Rat, Cow, Dog, Horse and Monkey.

Quick Overview for TLE2 antibody (AA 201-250) (ABIN6737675)

Target: TLE2 (Transducin-Like Enhancer Protein 2 (TLE2))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TLE2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TLE2 Antibody

Binding Specificity: AA 201-250

Specificity: Human TLE2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Bovine, Horse (100%) Mouse, Rat (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa201-250 of human TLE2 (Q04725, NP_003251). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Marmoset, Elephant, Dog, Bovine, Horse (100%), Mouse, Rat, Zebra finch (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for TLE2

Target: TLE2 (Transducin-Like Enhancer Protein 2 (TLE2))

Alternative Name: TLE2

Background: Name/Gene ID: TLE2

Synonyms: TLE2, ESG, Enhancer of split groucho 2, ESG2, GRG2

Gene ID: 7089

NCBI Accession: NP_003251

UniProt: Q04725

Pathways: WNT Signaling