PCK1 antibody (N-Term)

Catalog No. ABIN6737695

Product Details anti-PCK1 Antibody

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Binding Specificity: N-Term

Reactivity: Human, Cow, Rabbit, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCK1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human PCK1

Predicted Reactivity: Percent identity by BLAST analysis: Bovine (100%) Mouse (92%) Rabbit, Pig (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human PCK1 (P35558, NP_002582). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Bovine (100%), Orangutan, Galago, Marmoset, Mouse, Hamster, Bat, Guinea pig (92%), Monkey (85%), Rat, Elephant, Panda, Rabbit, Horse, Pig (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PCK1

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Alternative Name: PCK1 (PCK1 Products)

Background: Name/Gene ID: PCK1

Synonyms: PCK1, PEPCKC, Phosphopyruvate carboxylase, PEP carboxykinase, PEPCK-C, PEPCK1

Gene ID: 5105

NCBI Accession: NP_002582

UniProt: P35558

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis