HNF1B antibody (AA 38-87)

Catalog No. ABIN6737705

The Rabbit Polyclonal anti-HNF1B antibody has been validated for WB. It is suitable to detect HNF1B in samples from Human, Rat, Mouse, Cow, Horse, Pig, Bat and Monkey.

Quick Overview for HNF1B antibody (AA 38-87) (ABIN6737705)

Target: HNF1B (HNF1 Homeobox B (HNF1B))

Reactivity: Human, Rat, Mouse, Cow, Horse, Pig, Bat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HNF1B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HNF1B Antibody

Binding Specificity: AA 38-87

Specificity: Human HNF1B / TCF2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Bovine, Horse, Pig (100%) Dog, Guinea pig (92%) Zebrafish (90%) Mouse, Rat (85%) Xenopus (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa38-87 of human HNF1B (P35680, NP_000449). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Elephant, Bovine, Bat, Horse, Pig, Opossum (100%), Dog, Guinea pig (92%), Stickleback, Zebrafish (90%), Mouse, Rat (85%), Xenopus (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for HNF1B

Target: HNF1B (HNF1 Homeobox B (HNF1B))

Alternative Name: HNF1B / TCF2

Background: Name/Gene ID: HNF1B
Family: Transcription factor

Synonyms: HNF1B, FJHN, HNF-1B, HNF1 homeobox B, HNF2, HPC11, LF-B3, LFB3, HNF1beta, TCF2, MODY5, VHNF1, HNF-1-beta, HNF1 beta A, Homeoprotein LFB3, TCF-2, Transcription factor 2

Gene ID: 6928

NCBI Accession: NP_000449

UniProt: P35680

Pathways: Hormone Transport, Stem Cell Maintenance, Tube Formation