UBE2M antibody (N-Term)

Catalog No. ABIN6738052

This anti-UBE2M antibody is a Rabbit Polyclonal antibody detecting UBE2M in WB. Suitable for Human, Mouse, Xenopus laevis, Rat, Dog, Cow, Guinea Pig, Horse, Monkey and Rabbit.

Quick Overview for UBE2M antibody (N-Term) (ABIN6738052)

Target: UBE2M (ube2m) (Ubiquitin-Conjugating Enzyme E2M (ube2m))

Reactivity: Human, Mouse, Xenopus laevis, Rat, Dog, Cow, Guinea Pig, Horse, Monkey, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2M antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-UBE2M Antibody

Binding Specificity: N-Term

Specificity: Human UBE2M

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Rat, Dog (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human UBE2M (P61081, NP_003960). Percent identity by BLAST analysis: Human, Chimpanzee, Monkey, Galago, Marmoset, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Guinea pig, Xenopus (100%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for UBE2M

Target: UBE2M (ube2m) (Ubiquitin-Conjugating Enzyme E2M (ube2m))

Alternative Name: UBE2M / UBC12

Background: Name/Gene ID: UBE2M

Synonyms: UBE2M, NEDD8 protein ligase, NEDD8 carrier protein, NEDD8-conjugating enzyme Ubc12, HUbc12, UBC-RS2, UBC12, Ubiquitin-protein ligase M, Yeast UBC12 homolog, Ubiquitin carrier protein M

Gene ID: 9040

NCBI Accession: NP_003960

UniProt: P61081