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RUFY1 antibody (C-Term)

The Rabbit Polyclonal anti-RUFY1 antibody has been validated for WB. It is suitable to detect RUFY1 in samples from Human, Mouse, Rat, Dog, Rabbit, Guinea Pig, Zebrafish (Danio rerio), Monkey, Bat, Chicken and Pig.
Catalog No. ABIN6738071

Quick Overview for RUFY1 antibody (C-Term) (ABIN6738071)

Target

See all RUFY1 Antibodies
RUFY1 (RUN and FYVE Domain Containing 1 (RUFY1))

Reactivity

Human, Mouse, Rat, Dog, Rabbit, Guinea Pig, Zebrafish (Danio rerio), Monkey, Bat, Chicken, Pig

Host

  • 26
  • 4
  • 1
Rabbit

Clonality

  • 29
  • 2
Polyclonal

Conjugate

  • 24
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This RUFY1 antibody is un-conjugated

Application

  • 23
  • 15
  • 5
  • 4
  • 4
  • 3
  • 1
  • 1
Western Blotting (WB)
  • Binding Specificity

    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term

    Specificity

    Human RUFY1

    Predicted Reactivity

    Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit, Pig, Guinea pig, Chicken (100%) Horse, Xenopus (92%).

    Purification

    Immunoaffinity purified

    Immunogen

    Synthetic peptide from C-Terminus of human RUFY1 (A8K7B1, NP_001035542). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bat, Rabbit, Pig, Guinea pig, Zebra finch, Chicken, Platypus, Lizard, Zebrafish (100%), Horse, Opossum, Xenopus, Stickleback (92%), Orangutan, Monkey, Panda, Bovine (84%).

    Type of Immunogen: Synthetic peptide

    Isotype

    IgG
  • Application Notes

    Approved: WB

    Usage: ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

    Comment

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Distilled Water.

    Concentration

    Lot specific

    Buffer

    Lyophilized from PBS with 2 % sucrose

    Handling Advice

    Avoid repeat freeze-thaw cycles.

    Storage

    4 °C,-20 °C

    Storage Comment

    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target

    RUFY1 (RUN and FYVE Domain Containing 1 (RUFY1))

    Alternative Name

    RUFY1

    Background

    Name/Gene ID: RUFY1

    Synonyms: RUFY1, FYVE-finger protein EIP1, RABIP4, La-binding protein 1, Rab4-interacting protein, ZFYVE12

    Gene ID

    80230

    NCBI Accession

    NP_001035542

    UniProt

    Q96T51
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