PAM antibody (AA 146-195)

Catalog No. ABIN6738078

The Rabbit Polyclonal anti-PAM antibody has been validated for WB. It is suitable to detect PAM in samples from Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Bat, Monkey and Pig.

Quick Overview for PAM antibody (AA 146-195) (ABIN6738078)

Target: PAM (Peptidylglycine alpha-Amidating Monooxygenase (PAM))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Bat, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAM antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PAM Antibody

Binding Specificity: AA 146-195

Specificity: Human PAM

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Rabbit, Horse, Pig (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa146-195 of human PAM (A6NMR0, NP_000910). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig (100%), Zebra finch (91%), Turkey (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PAM

Target: PAM (Peptidylglycine alpha-Amidating Monooxygenase (PAM))

Alternative Name: PAL / PAM

Background: Name/Gene ID: PAM

Synonyms: PAM, PAL, Peptidylglycine 2-hydroxylase, Peptidylamidoglycolate lyase, PHM

Gene ID: 5066

NCBI Accession: NP_000910

UniProt: P19021