PDK3 antibody (AA 74-123)

Catalog No. ABIN6738214

This anti-PDK3 antibody is a Rabbit Polyclonal antibody detecting PDK3 in WB. Suitable for Human, Mouse, Rat, Cow, Guinea Pig, Dog, Horse, Rabbit, Pig, Hamster, Monkey and Xenopus laevis.

Quick Overview for PDK3 antibody (AA 74-123) (ABIN6738214)

Target: PDK3 (Pyruvate Dehydrogenase Kinase, Isozyme 3 (PDK3))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Dog, Horse, Rabbit, Pig, Hamster, Monkey, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDK3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PDK3 Antibody

Binding Specificity: AA 74-123

Specificity: Human PDK3

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Horse, Pig (100%) Chicken (92%) Guinea pig (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa74-123 of human PDK3 (Q15120, NP_005382). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Xenopus (100%), Turkey, Zebra finch, Chicken, Lizard (92%), Opossum (85%), Guinea pig, Catfish (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PDK3

Target: PDK3 (Pyruvate Dehydrogenase Kinase, Isozyme 3 (PDK3))

Alternative Name: PDK3

Background: Name/Gene ID: PDK3
Subfamily: PDK/BCKDK
Family: Protein Kinase

Synonyms: PDK3, PDHK3

Gene ID: 5165

NCBI Accession: NP_005382

UniProt: Q15120

Pathways: PI3K-Akt Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect